9am-noon, 1pm- 4pm, Sunday, July 21, 2019
no registration fee but you must register when you register for AMS https://twitter.com/NativeMassSpec
Native mass spectrometry is a growing area in the mass spectrometry community. Native MS is making significant contributions to structural biology. Because of its ease of application and broad applicability, native MS often leads and guides an integrated approach that eventually uses other structural biology tools. This pre-AMS workshop is sponsored by the NIH P41 “Native MS Guided Structural Biology”. The workshop is designed to introduce the broad field to new practitioners of native MS. Topics to be covered include sample preparation for native MS; instrumentation used for native MS – how does it differ from other MS; spray conditions to keep soluble complexes native; spray conditions for membrane protein complexes (micelles, liposomes, nanodiscs); activation methods for native MS (CID, CIU, SID, UVPD, ETD/ECD/EID); ion mobility for native MS; online separations approaches coupled to native MS (IEX, SEC, CE); software tools for native MS; case studies for protein:protein, protein ligand, RNA: protein, DNA:protein, and membrane protein complexes.
| Topic | Instructor |
|---|---|
| Instrumentation for native MS | Dave Russell/Vicki Wysocki |
| Sample Prep/online separations | Michal Sharon/Brandon Ruotolo |
| Spray and instrument conditions | Art Lagonowsky/Michael Marty |
| Ion mobility | Brandon Ruotolo/Dave Russell |
| Activation methods | Vicki Wysocki/Jim Prell |
| Data Analysis tools-MS&IM | Michael Marty/Jim Prell |
| Case studies | Michal Sharon/Art Lagonowsky |